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Lectin-peroxidase conjugates in histopathology of gastrointestinal mucosa
Wolf D. Kuhlmann, P. Peschke, K. Wurster
Laboratory of Experimental Medicine and Immunocytochemistry,
Institut für Nuklearmedizin, DKFZ Heidelberg, Germany
Institute of Pathology, City Hospital München-Schwabing, München, Germany
Peroxidase (HRP) conjugates of Dolichos biflorus agglutinin (DBA), Arachis hypogaea agglutinin (PNA) and Ulex europaeus agglutinin (UEA I) are used as specific molecular probes in order to describe histological patterns of carbohydrate moieties in glycoconjugates of gastric mucosa. Specimens of normal mucosa, metaplasias and carcinomas are fixed, embedded in paraffin and stained with lectins by a postembedding technique.