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Microscopy and stains in histology
Immune sera, Antibodies
Markers and conjugates
Tissue preparation
Immuno-staining, other ligand detection
Selection of staining protocols
Specificity, control reactions
Reagents, solutions
Laboratory methods
Applied cell labeling  

HomeCell-MarkerImmuno-staining, other ligand detection → Inhibition of endogenous enzyme activity

Inhibition of endogenous enzyme activity

Wolf D. Kuhlmann

Division of Radiooncology, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany

Nonspecific staining in immunohistology can be a multifactorial problem and may occur to variable degrees. Because the so-called “background” reactions will affect the interpretation of results, appropriate controls are necessary to recognize the underlying reasons and to find out solutions to prevent nonspecificity. In the case of enzymes as markers, endogenous enzyme activities (e.g. peroxidases, alkaline phosphatases) should be blocked to avoid interference with specific immunostaining.