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Microscopy and stains in histology
Immune sera, Antibodies
Markers and conjugates
Tissue preparation
Immuno-staining, other ligand detection
Selection of staining protocols
Specificity, control reactions
Reagents, solutions
Laboratory methods
Applied cell labeling  

HomeCell-MarkerSelection of staining protocols → Immunofluorescence assay for autoantibodies to nuclear antigens (ANA) on HEp-2 cells

Wolf D. Kuhlmann

Labor - Diagnostik - Beratung, D-56112 Lahnstein, Germany
MVZ für Laboratoriumsmedizin Koblenz-Mittelrhein, D-56068 Koblenz, Germany

Anti-nuclear antibody (ANA) testing by indirect immunofluorescence (IFT) is a fundamental laboratory method for the detection of antibodies in autoimmune diseases such as systemic autoimmune rheumatic diseases (SARD) from which clinical entities such as lupus erythematosus, mixed connective tissue disease, Sjögren’s syndrome, systemic sclerosis, polymyositis, dermatomyositis and overlap syndromes are typical examples. ANA is a hallmark for diagnosing and differentiating such diseases.