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Microscopy and stains in histology
Immune sera, Antibodies
Markers and conjugates
Tissue preparation
Immuno-staining, other ligand detection
Selection of staining protocols
Specificity, control reactions
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HomeCell-MarkerLaboratory methods → Toluidine blue staining

Toluidine blue staining

Wolf D. Kuhlmann

Division of Radiooncology, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany

Subsequent to immunohistological reaction, counterstaining of tissue sections with the thiazin dye toluidine blue (Toluidine blue O) is an alternative to other dyes (e.g. haematoxylin, carmine, methyl green) for nuclear staining. Moreover, the staining procedure is rapid and easily performed, thus, toluidine blue is helpful and widely employed for any kind of overview stainings. There exist formulations for cryostat and paraffin sections as well as for sections cut from resin embedded tissue.