Division of Radiooncology, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany
The use of sublimate in tissue fixation (e.g. Zenker solution) will lead to irritating salt precipitations in histological preparations which must be eliminated prior to microscopy. The elimination procedure is called “De-Zenkerization” and can be performed with tissue blocks just after fixation and prior to paraffin embedding or with deparaffinated tissue sections (e.g. prior or after immunostaining).