Cellular localization of lectin-affinity in tissue sections of normal human duodenum
K. Wurster, P. Peschke, Wolf D. Kuhlmann
Institut für Pathologie, Städtisches Krankenhaus München-Schwabing, D-8000 München, Germany
Labor für Experimentelle Medizin, Institut für Nuklearmedizin DKFZ, D-6900 Heidelberg, Germany
Peroxidase (HRP) conjugates of Arachis hypogaea agglutinin (PNA), Dolichos biflorus (DBA), Ulex europaeus agglutinin I (UEA I) and Bandeiraea simplicifolia agglutinin I (BSA I) were used to detect the expression of carbohydrate moieties in glycoconjugates of normal human duodenal mucosa. Lectin histology was performed on sections from paraffin embedded specimens of donor blood groups A, B, AB and 0. While PNA, DBA and UEA I staining appeared to be independent of the donor blood groups, BSA I affinity was restricted to the blood groups B and AB. Neither striated columnar cells nor goblet cells stained with PNA-HRP conjugates. Glycoconjugates of goblet cell mucins reacted with DBA and UEA I, but staining intensities varied in crypts and villi: positive goblet cells occurred preferentially in upper parts of the crypts and in villi. Striated columnar cells also exhibited variation in staining intensities, with binding of DBA and UEA I observed in the upper half of crypts and in villi, occurring predominantly along the brush border and in apical parts of the cells. Glycoconjugates synthesized by Brunnerís gland cells possessed a great variety of lectin reactivity with the lectins employed. Within a given histological section positive gland areas with different staining intensities beside negative cell populations might reflect different functional states.